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Journal: PLoS ONE
Article Title: H4 Histamine Receptors Mediate Cell Cycle Arrest in Growth Factor-Induced Murine and Human Hematopoietic Progenitor Cells
doi: 10.1371/journal.pone.0006504
Figure Lengend Snippet: (A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle Oligo GEArray. Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Article Snippet: TrueLabeling-AMP TM Linear RNA Amplification Kit and
Techniques: Expressing, Isolation, Staining, Incubation, Western Blot