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Qiagen oligo gearray® mouse cell cycle microarray (omm-020)
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Oligo Gearray® Mouse Cell Cycle Microarray (Omm 020), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen rt2 microarray composed cell cycle genes
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
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Qiagen human cell cycle rt 2 -pcr microarrays
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Human Cell Cycle Rt 2 Pcr Microarrays, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CapitalBio Corporation human cell cycle genome oligonucleotide microarrays
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Human Cell Cycle Genome Oligonucleotide Microarrays, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen human cell cycle (ohs-020) oligo gearray dna microarrays
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
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Qiagen rt 2 profiler pcr microarray cell cycle phas-020
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
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Qiagen mouse cell cycle microarray plates
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Mouse Cell Cycle Microarray Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Full Moon BioSystems cell cycle phospho antibody microarray
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Cell Cycle Phospho Antibody Microarray, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Full Moon BioSystems cell cycle control/dna damage phospho-antibody microarray
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Cell Cycle Control/Dna Damage Phospho Antibody Microarray, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen rt2 profiler pcr microarray (cell cycle (phas020)
(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle <t>Oligo</t> <t>GEArray.</t> Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.
Rt2 Profiler Pcr Microarray (Cell Cycle (Phas020), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle Oligo GEArray. Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.

Journal: PLoS ONE

Article Title: H4 Histamine Receptors Mediate Cell Cycle Arrest in Growth Factor-Induced Murine and Human Hematopoietic Progenitor Cells

doi: 10.1371/journal.pone.0006504

Figure Lengend Snippet: (A) The effect of 10 −5 M CB on the expression of cell cycle genes was assessed in freshly isolated c-kit + BM cells and after a 3-h exposure to growth factor cocktail (GF) with or without CB, using a cell cycle Oligo GEArray. Fold decrease upon exposure to CB: 35 for cyclin D3: and: 54 for cyclin E. (B) Decreased intracellular staining with FITC-conjugated anti-cyclin D3 antibody after an overnight incubation of sorted c-kit + cells in the presence of CB, as compared with growth factors alone. Data represent a typical dot plot and means±SEM from 3 experiments. (C) Western blot analysis in whole-cell extracts before and after a 5-h incubation of sorted c-kit + cells with growth factors alone or together with CB.

Article Snippet: TrueLabeling-AMP TM Linear RNA Amplification Kit and Oligo GEArray® Mouse cell cycle Microarray (OMM-020) were purchased from SABiosciences (tebu-bio, France) The following appropriately labeled antibodies were used: anti-mouse CD117 (c-kit) (2B8), TCRβ (H57-597), Ly-6G(Gr-1) (RB6-8C5), CD11b (M1/70), CD45R(B220) (RA3-6B2), CD4 (H129.19), CD8 (53–6.7), CD19 (1D3), TER-119, Ly-6A/E (Sca-1) (D7) and anti-human CD34 (581) (all from Pharmingen, San Diego, CA).

Techniques: Expressing, Isolation, Staining, Incubation, Western Blot